The Fridman lab aims to elucidate the mechanisms of antimicrobial activity and develop novel concepts for antimicrobial drug development, targeting both bacterial and fungal pathogens. Recognizing the diverse cellular architectures and intracellular target localizations across bacterial and fungal pathogens, we employ synthetic modifications to regain drug interactions with targets that have undergone resistance-conferring mutations. Utilizing advanced imaging techniques and by developing unique fluorescent antimicrobial probes, we investigate the molecular determinants of antimicrobial uptake and subcellular distribution. This approach allows us to define the relationship between drug localization and efficacy, and to explore how these factors influence resistance, tolerance, and mode/s of action.